ROS-Nitric Oxide Pathway Reagents and Kits
Enzo Life Sciences ROS-ID ROS/RNS detection kit (1Kit)
Enzo Life Sciences’ ROS-ID ROS/RNS detection kit is designed to directly monitor real time reactive oxygen and/or nitrogen species (ROS/RNS) production in live cells using fluorescence microscopy. The kit includes three fluorescent dye reagents: NO Detection Reagent (red fluorescent), Oxidative Stress Detection Reagent (green fluorescent) for total ROS detection, and Superoxide Detection Reagent (orange fluorescent). It also includes the positive controls, pyocyanin and L-arginine, which are common inducers of ROS and NO production, respectively, as well as the negative controls, N-acetyl-L-cysteine and c-PTIO (common scavengers of ROS and NO, respectively). Through the combination of the three detection probes with a set of specific inhibitors and activators, this kit enables discrimination among superoxide, nitric oxide and peroxynitrite. Applications: Fluorescence microscopy .
Enzo Life Sciences DETA NONOate (25mg). CAS: 146724-94-9
Nitric oxide (NO) donor. Induces apoptosis in macrophages. The half-life time of DETA NONOate is 20 hours at 37°C and 56 hours at 22-25°C in 0.1M phosphate buffer, pH 7.4. Decomposition of NONOates is nearly instantaneous at pH 5.0. DETA NONOate liberates 2 mol of NO per mol of parent compound. Alternative name: NOC-18, DETA/NO, (Z)-1-[2-(2-Aminoethyl)-N-(2-ammonioethyl)amino]diazen-1-ium-1,2-diolate, 3 3-Bis(aminoethyl)-1-hydroxy-2-oxo-1-triazene, 2 2'-(Hydroxynitrosohydrazino)bis-ethanamine. Purity: ≥97% (1H-NMR). Solubility: Soluble in water or ethanol. Long Term Storage: -80°C.
Enzo Life Sciences DEA NONOate (50mg). CAS: 372965-00-9
Nitric oxide (NO) donor. Alternative name: DEA/NO, 2-(N,N-Diethylamino)-diazenolate-2-oxide . diethylammonium salt. Purity: ≥97% (NMR). Solubility: Soluble in 100% ethanol (25mg/ml), DMSO (2mg/ml), dimethyl formamide (2mg/ml) or PBS, pH 7.2 (10mg/ml). Long Term Storage: -80°C.
Sigma Aldrich Fine Chemicals Biosciences TranscreenerR ADP2 fI Assa
The Transcreener(R) HTS Assay platform overcomes the need for time-consuming one-off assay development for individual members within a group transfer enzyme family by utilizing a single set of assay reagents that detect an invariant product. The generic nature of the Transcreener(R) HTS Assay platform eliminates delays involved in assay development for new HTS targets and greatly simplifies compound and inhibitor profiling across multiple target families. The Transcreener(R) ADP2 fluorescent intensity (fI) assay extends the Transcreener(R) platform for ADP detection by utilizing a simple fluorescent intensity output which can be used on both fluorescence readers typically found in academic and therapeutic research labs as well as more complex multimode plate readers more commonly used in core facilities and HTS labs. The Transcreener(R) ADP2 fI Assay is a red competitive fluorescence intensity (fI) assay based on the detection of ADP and therefore is compatible with any enzyme class
Sigma Aldrich Fine Chemicals Biosciences TranscreenerR ADP2 FI Assa
The Transcreener(R) HTS Assay platform overcomes the need for time-consuming one-off assay development for individual members within a group transfer enzyme family by utilizing a single set of assay reagents that detect an invariant product. The generic nature of the Transcreener(R) HTS Assay platform eliminates delays involved in assay development for new HTS targets and greatly simplifies compound and inhibitor profiling across multiple target families. The Transcreener(R) ADP2 fluorescent intensity (FI) assay extends the Transcreener(R) platform for ADP detection by utilizing a simple fluorescent intensity output which can be used on both fluorescence readers typically found in academic and therapeutic research labs as well as more complex multimode plate readers more commonly used in core facilities and HTS labs. The Transcreener(R) ADP2 FI Assay is a red competitive fluorescence intensity (FI) assay based on the detection of ADP and therefore is compatible with any enzyme class
Cayman Chemical DNARNA OxIdatIve DamageHIg 96
An ALDH1A3 inhibitor (Ki 0.55 UM) selective for ALDH1A3 over ALDH1A1 (Ki 78.2 UM) inhibits the conversion of retinol to retinaldehyde in U87MG cells at 15 UM reduces the viability of GSC-83 and GSC-326 cells at 15 UM
Cayman Chemical CreatInInurInary ColorIme 480
A mitochondrial protein import inhibitor inhibits the import of cytochrome c1 into the mitochondrial intermembrane space in yeast cells at 100 UM inhibits migration and invasion of and induces apoptosis and cell cycle arrest at the G1 phase in primary human bladder cancer cells at 25 UM induces mitochondrial depolarization decreases ATP levels and increases ROS in primary human bladder cancer cells decreases tumor volume in a PDX mouse model of bladder cancer at 20 mg/kg every other day intravitreal administration reduces SOD activity and increases TBARS levels and acellular capillary numbers in mouse retina at 0.25 nM/eye induces dorsal curvature and cardiac apoptosis in zebrafish embryos at 10 UM
Sigma Aldrich Fine Chemicals Biosciences Tyrosine Colorimetric Assa
Tyrosine (Tyr) is an aromatic amino acid that is a precursor of several important substrates such as catecholamine thyroid hormone and melanin pigment. A deficiency in enzymes such as homogentisic acid oxidase or phenylalanine 4-hydroxylase that degrade tyrosine result in rare hereditary metabolic disorders.
Sigma Aldrich Fine Chemicals Biosciences Colorimetric Aldehyde Assa
Aldehydes are highly reactive molecules which play a role in many physiological pathological and commercial processes. They have been implicated as environmental pollutants and in neurodegeneration and DNA damage. A method for rapid and accurate measurement of aldehydes is an important tool for biological and chemical research the food industry and environmental pollution surveillance.
Cayman Chemical PhenylarsIn OxIde 1g
A typical antipsychotic binds to the dopamine D1 receptor and androgen receptor (Kis 0 01 and 3 UM respectively) an a1-AR antagonist (IC50 4 1 nM in rat forebrain homogenates) an a2-AR antagonist (IC50 2 UM in rat cortical homogenates) inhibits SARS-CoV-2 Mpro activity by 55 at 100 UM increases the number of entries into and the percentage of time spent in the open arms of the elevated plus maze in rats at 0 075 mg/kg
Cayman Chemical AlkalIn Phosphatse ColorIme
An antagonist of the androgen receptor NTD inhibits androgen receptor NTD transactivation in a reporter assay (IC50 7.4 UM) reduces androgen-dependent growth of LNCaP cells from 10-30 UM decreases tumor volume in an LNCaP CRPC mouse xenograft model at 50 mg/kg
Cayman Chemical ROS DetectIon CelBased96
A mixture of dihydroartemisinin isomers contains a-dihydroartemisinin andBdihydroartemisinin
Abcam Alkaline Phosphatase Assay Kit (Fluorometric)
Alkaline Phosphatase Assay Kit (Fluorometric)
The product is subject to the following: Abcam Restricted Use Statement